Synthesis of Ethanolamine and Its Regulation in Lemna paucicostata

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Uptake of Choline and Ethanolamine by Lemna paucicostata Hegelm. 6746.

Lemna paucicostata Hegelm. 6746 possesses specific systems for uptake of choline and ethanolamine. Each is distinct from the six other systems for uptake of organic compounds so far identified in this plant. Both systems show biphasic kinetics, so that uptake by them can be described as the composite result of two Michaelis-Menten processes. Inhibitor studies are reported which indicate the ver...

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Synthesis of methylated ethanolamine moieties: regulation by choline in lemna.

The results of experiments in which intact plants of Lemna paucicostata were labeled with either l-[(3)H(3)C]methionine, l-[(14)CH(3)]methionine, or [1,2-(14)C]ethanolamine support the conclusion that growth in concentrations of choline of 3.0 micromolar or above brings about marked decreases in the rate of biosynthesis of methylated forms of ethanolamine (normally present chiefly as phosphatid...

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Threonine Synthase of Lemna paucicostata Hegelm. 6746.

Threonine synthase (TS) was purified approximately 40-fold from Lemna paucicostata, and some of its properties determined by use of a sensitive and specific assay. During the course of its purification, TS was separated from cystathionine gamma-synthase, establishing the separate identity of these enzymes. Compared to cystathionine gamma-synthase, TS is relatively insensitive to irreversible in...

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Aspartokinase of Lemna paucicostata Hegelm. 6746.

A sensitive and specific method was developed for assay of aspartokinase (EC 2.7.2.4) in crude extracts of Lemna paucicostata. Lysine inhibited approximately 93%, and threonine approximately 6%; together, these amino acids inhibited 99%. Inhibition by lysine was synergistically increased by S-adenosylmethionine, which by itself had no effect on activity. Essentially complete inhibition of threo...

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C14-oxylipin glucosides isolated from Lemna paucicostata.

Oxylipin glucosides (2-4) were isolated from Lemna paucicostata with their structures and absolute configurations elucidated by spectroscopic and chemical methods. Compounds 2-4 were glucosides of C14 oxylipin which were synthesized from alpha-linolenic acid via the 9-lipoxygenase pathway.

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1989

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.91.2.587